The 41-year-old Australian singer and 'Get Busy' hitmaker beat off stiff competition from the list of 20 artists including Justin Bieber's 'Love Yourself', Calvin Harris and Rihanna's 'This is What You Came For' and Beyoncé's 'Sorry'.
Meanwhile, Jonas Blue had two tracks on the list, 'Perfect Strangers' and 'Fast Car'.
Talking about his successful year, which saw him gain over a billion streams, he exclusively told BANG Showbiz: ''It has been a bit mad, a billion streams now, it's been nuts.''
Asked what has been the highlight, he added: ''probably that. Doing that and being able to have this amazing platform where I can just share all my music around the world and do what I love.''
Elsewhere on the list is Coldplay and Beyoncé's 'Hymn For The Weekend', DNCE's 'Cake By The Ocean', Fifth Harmony's 'Work from Home' featuring Ty Dolla $ign and Justin Timberlake's 'Can't Stop The Feeling'.
And Lukas Graham's 7 Years', Mike Posner's comeback single 'I Took A Pill in Ibiza', Shawn Mendes 'Stitches', The Chainsmokers and Daya's 'Don't Let Me down', Zara Larsson's 'Lush Life', Zayn's 'Pillowtalk' and Twenty One Pilots 'Stressed Out'.
MTV's Top 20 Most Played Music Videos of 2016 are as follows:
Alan Walker - 'Faded'
Beyoncé - 'Sorry'
Calvin Harris Ft. Rihanna - 'This is What You Came For'
Coldplay Ft. Beyonce - 'Hymn For The Weekend'
DJ Snake Ft. Justin Bieber - 'Let Me Love You'
DNCE - 'Cake By The Ocean'
Fifth Harmony Ft. Ty Dolla $ign - 'Work from Home'
Jonas Blue Ft. Dakota - 'Fast Car '
Jonas Blue Ft. JP Cooper - 'Perfect Strangers '
Justin Bieber - 'Love Yourself '
Justin Timberlake - 'Can't Stop The Feeling'
Kungs Vs Cookin On 3 Burners - 'This Girl'
Lukas Graham - '7 Years'
Mike Posner - 'I Took A Pill In Ibiza '
Shawn Mendes - 'Stitches'
The Chainsmokers Ft. Daya - 'Don't Let Me Down'
Twenty One Pilots - 'Stressed Out '
Zara Larsson - 'Lush Life'
Zayn - 'Pillowtalk'
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