The Borat funnyman had been set to play flamboyant frontman Freddie Mercury in the film he was helping to develop, but he walked away from the project in 2013.
The British actor has since claimed he quit over artistic differences, revealing he clashed with the surviving Queen members over how the movie should depict the more salacious aspects of the late singer's rock 'n' roll lifestyle.
"They wanted to protect their legacy as a band," he said during a candid radio interview on The Howard Stern Show last month (Mar16).
Cohen also disagreed with the musicians' plans to show Mercury's death halfway through the film, and then shift the focus onto how Queen continued on after the Bohemian Rhapsody singer's loss in 1991.
However, Cohen's comments have enraged May.
"Sacha became an a**e," the guitar great fumed to Britain's Mail on Sunday. "We had some nice times with Sacha kicking around ideas, but he went off and told untruths about what happened. Why would he go away and say that we didn't want to make a gritty film?"
May goes on to suggest the comedian didn't quit - he was fired.
"We decided he wasn't right for the role for very good reasons, which will become apparent if you watch what he's done recently," May said, taking a shot at his box office flop Grimsby. "It's obvious that it wasn't going to work, him playing Freddie. It wouldn't suspend your disbelief."
Skyfall actor Ben Whishaw subsequently emerged as the frontrunner to portray Mercury, but the biopic stalled until November (15), when writer Anthony MCCarten was tapped to pen a new script.
"We're hoping Ben Whishaw will do it," May added, before aiming another dig at Cohen. "He's fabulous - a real actor."