The Dominican-American heartthrob will take the stage to sing his latest English-language single, Stuck On A Feeling, as well as his Spanish-language hit song Darte Un Beso.
Meanwhile, Jonas will perform his hit song Jealous. The former Jonas Brothers star has a special connection with the annual pageant - his girlfriend Olivia Culpo was crowned Miss Universe in 2012.
The beauty contest will also have a star-studded judging panel, including telenovela actor William Levy, reality Tv stars Kristin Cavallari and Lisa Vanderpump, and Filipino boxer Manny Pacquiao.
The 63rd Annual Miss Universe pageant will air live from Doral, Florida on 25 January (15).