The 'Hard Out Here' singer has stepped in to replace the band after it was reported that frontman Alex Trimble was in hospital and unable to perform the band's headline slot at the music event at Henham Park in Southwold, Suffolk, in Eastern England on Friday (18.07.14) night.
She said in a statement: ''So sorry to hear about Alex from Two Door Cinema Club. However, I am more than happy to step in. I've never been to Latitude, but I hear it's fabulous and I can' wait to get my heels on for some serious dancing action on Friday evening - so, so excited.''
In addition to adding Lily to the line-up, festival organisers are also planning to make up for the loss of the 'Changing of the Seasons' singers by promising fans a surprise guest performance on the night.
Managing Director of Festival Republic Melvin Benn said of the change in line-up: ''We are honoured that Lily Allen is able to step in at the last minute and would like to thank her for filling the headline slot. After her show-stopping performance at Glastonbury Lily was a natural choice, the Latitude audience are in for something really spectacular.''
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Pratchett died aged 66 in March 2015.