During the first headline set of the weekend at the Empire Polo Club in Indio, California, the rockers covered Bowie's Heroes during their set, as well as playing a snippet of November Rain by Saturday's headliners GUNS N' ROSES.
The reunited Brooklyn band, who last toured in 2011, played a hit-laden set to the wild crowd, including I Can Change, Tribulations and You Wanted a Hit. They sampled November Rain as they performed their track New York, I Love You But You're Bringing Me Down, in homage to their fellow reunited headliners.
Frontman James Murphy told the crowd that they had decided to cut down on talking during their set so they could fit more tracks in.
"The last time we played here, Jay Z played after us and we were honoured because we really like him, " Murphy said of their Coachella performance in 2010. "But tonight he's not here, and we're sorry."
Towards the end of their set, he wearily announced "Two more," as they launched into a stirring rendition of Heroes in tribute to iconic musician Bowie, who died in January (16).
Prior to his death, Murphy worked with Bowie, remixing his track Love Is Lost in 2013.
The rockers released their last album, This is Happening, back in 2010 before breaking up. They reunited and played their first live show in nearly five years back in March (16).