The 'CBS Evening News' anchor will appear as herself in the musical drama series' post-Superbowl episode, which will air on February 6.
Although her storyline is yet to be confirmed, Katie will appear in scenes with cheerleading coach Sue Sylvester, who is played Jane Lynch.
The 53-year-old journalist has admitted she is a big fan of the show, as are her daughters.
She previously said: "It's something my 14-year-old daughter will actually do with me these days and we both marvel at how beautifully the show has tackled difficult subjects like being gay, death, single parenthood, teenage pregnancy, disabilities and faith to name a few."
Katie is set to interview 'Glee' heartthrob Matthew Morrison - who plays Spanish teacher and glee club director Will Schuester - on 'CBS News Sunday Morning' this weekend.