Hours before the Tony Awards on Sunday (12Jun16), a gunman shot and killed 49 people and injured another 53 at gay nightclub Pulse, in what has become the worst mass shooting in U.S. history.

While many attendees and winners at Broadway's biggest night paid tribute to those affected in the tragedy, many are continuing their support by recording a cover of Burt Bacharach and Hal David's 1965 hit What the World Needs Now Is Love for the cause.

More than 30 Broadway A-listers have already signed up to be part of the tribute track - in addition to Menzel, Miranda and MCDonald, others slated to contribute their talents include Sarah Jessica Parker, Matthew Broderick, Sara Bareilles, Carole King, Chita Rivera, Rosie O'Donnell, Kristen Bell, Bernadette Peters, Gloria Estefan, Sutton Foster, and Sean Hayes.

The stars recorded their parts in studios in New York, Los Angeles and Miami on Wednesday (15Jun16), and a music video will be released on Friday (17Jun16). The track will be available for purchase on Monday (20Jun16), and 100 per cent of the proceeds from sales will go to the LGBT Center of Central Florida.

Creator of musical Disaster! Seth Rudetsky and his producer husband James Wesley are spearheading the project, and were inspired by 1985 charity single We Are The World to quickly rally their famous friends together for the song.

"The Broadway community was so terribly shaken and devastated by the horrific tragedy in Orlando, everyone wanted to do something as quickly as possible that would truly make a difference," Rudetsky said in a statement. "We started making calls, and suddenly we had tons of theatre actors along with an orchestra and the services of a fully-staffed recording studio, all willing to donate their time and talent.

"Our community of artists has banded together as we always do to show we can end this cycle of violence and intolerance. Love WILL prevail!"

During Sunday's Tony Awards, winners such as Hamilton creator Miranda and veteran actor Frank Langella paid tribute to the Orlando victims, while host James Corden kicked off the night by addressing the tragedy.

"Our hearts go out to all of those affected by this atrocity," he began. "All we can say is you are not on your own right now. Your tragedy is our tragedy. Theatre is a place where every race, creed, sexuality, and gender is equal, is embraced, and is loved. Hate will never win. Together we have to make sure that tonight's show as a symbol and a celebration of that principle."