Little Eisele Kaye, three, ran out onstage into her mother's arms and waved to the crowd just before the trio performed American Honey at the Fraze Pavilion.
"Can you tell everybody your name?" the singer asked her daughter, who was suddenly overcome with shyness and buried her head in her mother's chest.
"Well, this is Eisele Kaye and she just turned three a couple weeks ago," Hillary told fans. "Last night at our show she was begging to come out on stage, so now of course she gets shy. Can you wave again?"
Eisele waved to the crowd and then offered up her own adorable rendition of the Alphabet Song.
Her proud mum posted a clip of her daughter's live singing debut on Instagram, adding the caption: "My precious girl made her Lady A stage debut tonight in Ohio!!! She did a PERFECT rendition of the ABCs before we sang American Honey! My. Life. Is. Made. Now."
Scott recently reunited with her bandmates after the release of her family-themed debut gospel album, Love Remains, which topped the Billboard Top Christian Albums chart this week.
The Lady Antebellum star co-wrote the album’s lead single Thy Will, which is currently the number one song on Billboard‘s Hot Christian Songs chart, after suffering a miscarriage last year (15).
She recently told Taste of Country, "It was my honest conversation with God: 'Why do bad things happen? But ultimately I trust your will for my life and that it is all going to be OK'. To be able to write that song was such a healing process for me."
Hillary, who is married to Chris Tyrrell, revealed the news about her miscarriage in June (16).