The 80s pop supergroup performed a mash-up of their 1981 hit Planet Earth and Bowie's Space Oddity during the show at the Barclays Center in Brooklyn.
Frontman Simon Le Bon has always been a big Bowie fan, once revealing he was "God to me".
In a 2005 interview, the Rio singer said, "I love everything he's done. He was so important to me growing up, and helped me towards a sense of identity."
Duran Duran even toured with the Let's Dance hitmaker in 1987, and often cover his songs onstage.
Bowie lost his battle with cancer in January (16).
Meanwhile, Duran Duran also stunned fans at the Barclays Center show by introducing actress Lindsay Lohan, who performed the spoken-word segment she recorded on the band's new Danceophobia track.