The opera singer-turned-pop star and Lucy Rose have been confirmed for Festival No. 6, which will take place in Portmeirion, Wales at the beginning of next month (Sep16).
The duo will be joined by Jacqui Abbott and Nadine Shah. The quartet will perform Bowie's hits, backed by the Manchester Camerata Orchestra.
Composer-in-residence Joe Duddell, who will conduct the show, told the BBC he invited the women to sing because he thought their take on Bowie's songs would be interesting.
"He (Bowie) was a very androgynous figure and, certainly in the early music, his voice is very high," Duddell explained. "If we were going to keep the songs in the same key, I was thinking that female singers would be the way forward - and we've stuck to that. It'll be fascinating to see what their interpretations of the songs will be."
Bastille, Hot Chip and Noel Gallagher's High Flying Birds will headline the festival.
Bowie lost his battle with cancer at the beginning of the year (Jan16).