The Call Me Maybe hitmaker was due to open for Bieber at his sold-out gig at the Consol Energy Center in Pittsburgh, Pennsylvania but she wasn't feeling well enough to perform.
In a post on Twitter.com, she told fans, "So bummed I'm missing the show. Sorry guys. Chicken noodle soup and bed for me."
Jepsen is next due to take the stage at Bieber's concert in Michigan on Wednesday (21Nov12) - her 27th birthday.