The opera star, who recently performed at the Winter Olympics Opening Ceremony in Sochi, Russia, has been rehearsing to play Marguerite in a revival of Charles Gounod's show in preparation of April's (13) opening.
However, the singer has since stepped down from the job and apologised for her withdrawal.
A statement released on Friday (28Feb14) reads: "I am so sorry to have to withdraw from the role of Marguerite in Faust. After much consideration and intensive preparation, I have come to the conclusion that the role is not right for me.
"I had been very much looking forward to debuting this role at the Royal Opera House and following it with further performances in Vienna (Austria) and Baden-Baden (Germany). Unfortunately, I must now withdraw from all these productions. I am very sad to be disappointing my fans in these cities and hope they will understand the difficult decision that I have had to make. However, I am very much looking forward to returning to The Royal Opera to perform with the company again in 2015."
A replacement for Netrebko has yet to be announced, although the rest of the line-up remains unchanged, with tenor Joseph Calleja in the title role and Bryn Terfel as Mephistopheles.